Canoe Creek State Park maintains a variety of habitats for wildlife. Old fields, wetlands, a lake, and a limestone deep mine are interspersed with deciduous woodlots, which provide excellent habitat for over 200 species of birds and mammals.
The park is a stopover resting spot for migrating birds and serves as a summer nesting site for others.
Canoe Lake is great for seeing migrating waterfowl.
The wetlands along Mary Ann's Creek are frequented by:
Osprey and bald eagle are noteworthy spring and fall migrants.
Because of Canoe Creek State Park’s excellent biodiversity and habitats, the park has been designated an Important Bird Area by the National Audubon Society and an Important Mammal Area by the Pennsylvania Game Commission.
Checklists and geocaching brochures can be obtained at the education center or the park office.
The Frank Felbaum Bat Sanctuary hosts a nursery colony of little brown bats. Historically an important colony due to its numbers, it has attracted attention of both park visitors and biologists over the years.
On warm summer nights, visitors attend programs and watch the emergence of scores of bats.
A gated limestone mine serves as a monitored hibernation site for several species of bats including state and federally endangered species.
Bat watching continues to be a popular summer activity for park visitors.